Correct regulation of transcription and accurate co-transcriptional mRNA processing is essential for gene expression.
Regulation of transcription is dependent on RNA polymerase II (RNAPII) modifications and its dynamic interaction with factors recognising specific modifications. Our work aims to understand the function of a class of Serine/Arginine (SR)-related and CTD-associated factors (SCAFs), which interact with the CTD of RNAPII.
Using a combination of molecular biology, state-of-the art transcriptomics and proteomics we aim to elucidate how transcription and co-transcriptional processing are regulated by RNAPII CTD associated factors.
This will provide new insights into the function of SCAF proteins in transcription – an area that is currently unexplored. Once we understand their role in transcription we can begin to understand how errors in transcriptional regulation and co-transcriptional processing contribute to disease development.